Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, COLO 320 cells, Jurkat cells, THP-1 cells, ROS1728 cells, HeLa cells, NIH/3T3 cells, HepG2 cells, K-562 cells, HSC-T6 cells, C6 cells, Raw 264.7 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 20 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
66278-1-Ig targets CDK6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CDK6 fusion protein Ag5600 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin-dependent kinase 6 |
| Calculated molecular weight | 326 aa, 36 kDa |
| Observed molecular weight | 36-40 kDa |
| GenBank accession number | BC027989 |
| Gene Symbol | CDK6 |
| Gene ID (NCBI) | 1021 |
| RRID | AB_2881661 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q00534 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK6 antibody 66278-1-Ig | Download protocol |
| IHC protocol for CDK6 antibody 66278-1-Ig | Download protocol |
| IF protocol for CDK6 antibody 66278-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Transcriptomics and molecular docking reveal the potential mechanism of lycorine against pancreatic cancer | ||
Int J Mol Sci (20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells. | ||
Cell Oncol (Dordr) LINC02154 promotes the proliferation and metastasis of hepatocellular carcinoma by enhancing SPC24 promoter activity and activating the PI3K-AKT signaling pathway. | ||
Oxid Med Cell Longev MICAL2 Contributes to Gastric Cancer Cell Proliferation by Promoting YAP Dephosphorylation and Nuclear Translocation | ||
Int J Oncol GBP1 promotes erlotinib resistance via PGK1‑activated EMT signaling in non‑small cell lung cancer. | ||
J Cancer Apolipoprotein L3 inhibits breast cancer proliferation and modulates cell cycle via the P53 pathway |