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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK6 Monoclonal antibody (66278-1-Ig)

    WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDK6 Monoclonal antibody (66278-1-Ig)

    WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CDK6 Monoclonal antibody (66278-1-Ig)

    WB analysis of HEK-293 using 66278-1-Ig (same clone as 66278-1-PBS)

    WB result of CDK6 antibody (66278-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HEK-293 cells. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDK6 Monoclonal antibody (66278-1-Ig)

    WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDK6 Monoclonal antibody (66278-1-Ig)

    WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using CDK6 Monoclonal antibody (66278-1-Ig)

    WB analysis of THP-1 using 66278-1-Ig (same clone as 66278-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CDK6 Monoclonal antibody (66278-1-Ig)

    WB analysis of Jurkat using 66278-1-Ig (same clone as 66278-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using CDK6 Monoclonal antibody (66278-1-Ig)

    WB analysis of NIH/3T3 using 66278-1-Ig (same clone as 66278-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CDK6 Monoclonal antibody (66278-1-Ig)

    IHC staining of human skin cancer using 66278-1-Ig (same clone as 66278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66278-1-Ig (CDK6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK6 Monoclonal antibody (66278-1-Ig)

    IF Staining of HeLa using 66278-1-Ig (same clone as 66278-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66278-1-Ig(CDK6 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CDK6 Monoclonal antibody, PBS Only

CDK6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66278-1-PBS
Clone No.4B9C11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 6, Cyclin dependent kinase 6, Cell division protein kinase 6, CDKN6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66278-1-PBS targets CDK6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDK6 fusion protein Ag5600 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 6
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC027989
Gene Symbol CDK6
Gene ID (NCBI) 1021
RRIDAB_2881661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00534
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).