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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK7 Monoclonal antibody

CDK7 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E7B6

Cat no : 67889-1-Ig

Synonyms

39 kDa protein kinase, CAK, CAK1, CDK activating kinase, CDK7, CDKN7, Cell division protein kinase 7, cyclin dependent kinase 7, MO15, p39 Mo15, p39MO15, STK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK7 Monoclonal antibody (67889-1-Ig)

    WB analysis using 67889-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using CDK7 Monoclonal antibody (67889-1-Ig)

    WB analysis using 67889-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDK7 Monoclonal antibody (67889-1-Ig)

    IHC staining of human breast cancer using 67889-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDK7 Monoclonal antibody (67889-1-Ig)

    IHC staining of human breast cancer using 67889-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK7 Monoclonal antibody (67889-1-Ig)

    IF Staining of HeLa using 67889-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CDK7 Monoclonal antibody (67889-1-Ig)

    IF Staining of A431 using 67889-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, U2OS cells, MCF-7 cells, Hela cells, Jurkat cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67889-1-Ig targets CDK7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDK7 fusion protein Ag25737 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 7
Calculated molecular weight 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC005298
Gene symbol CDK7
Gene ID (NCBI) 1022
RRIDAB_2918645
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CDK7 antibody 67889-1-IgDownload protocol
IHC protocol for CDK7 antibody 67889-1-IgDownload protocol
IF protocol for CDK7 antibody 67889-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols