CDK7 Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.