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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK8 Recombinant antibody (83621-6-RR)

    WB analysis using 83621-6-RR (same clone as 83621-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83621-6-RR (CDK8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83621-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK8 Recombinant antibody (83621-6-RR)

    IF Staining of HeLa using 83621-6-RR (same clone as 83621-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK8 antibody (83621-6-RR, Clone: 240598G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83621-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Caco-2 cells using CDK8 Recombinant antibody (83621-6-RR)

    FC experiment of Caco-2 using 83621-6-RR (same clone as 83621-6-PBS)

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug CDK8 Recombinant antibody (83621-6-RR, Clone:240598G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83621-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83621-6-RR

    Affinity and Kinetic Characterization of 83621-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83621-6-RR against Human CDK8 were performed. The affinity constant is 12.5 nM.

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CDK8 Recombinant antibody, PBS Only

CDK8 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83621-6-PBS
Clone No.240598G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8, Cell division protein kinase 8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83621-6-PBS targets CDK8 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK8 fusion protein Ag17148 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 8
Calculated molecular weight 464 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC069634
Gene Symbol CDK8
Gene ID (NCBI) 1024
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49336
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.