Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, A549 cells, HCT 116 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83621-6-RR targets CDK8 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CDK8 fusion protein Ag17148 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin-dependent kinase 8 |
| Calculated molecular weight | 464 aa, 53 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC069634 |
| Gene Symbol | CDK8 |
| Gene ID (NCBI) | 1024 |
| RRID | AB_3671234 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P49336 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK8 antibody 83621-6-RR | Download protocol |
| IF protocol for CDK8 antibody 83621-6-RR | Download protocol |
| FC protocol for CDK8 antibody 83621-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |