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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDK9 Recombinant antibody (83662-7-RR)

    IHC staining of human lung cancer using 83662-7-RR (same clone as 83662-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-7-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDK9 Recombinant antibody (83662-7-RR)

    IHC staining of human lung cancer using 83662-7-RR (same clone as 83662-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-7-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK9 Recombinant antibody (83662-7-RR)

    IF Staining of HeLa using 83662-7-RR (same clone as 83662-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK9 Recombinant antibody (83662-7-RR)

    IF Staining of HeLa using 83662-7-RR (same clone as 83662-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK9 Recombinant antibody (83662-7-RR)

    IF Staining of HeLa using 83662-7-RR (same clone as 83662-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83662-7-RR

    Affinity and Kinetic Characterization of 83662-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83662-7-RR against Human CDK9 were performed. The affinity constant is below 1 pM.

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CDK9 Recombinant antibody, PBS Only

CDK9 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 83662-7-PBS
Clone No.240600D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

CTK1, Cell division cycle 2-like protein kinase 4, CDC2L4, C-2K, C 2k

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83662-7-PBS targets CDK9 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK9 fusion protein Ag2318 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 9
Calculated molecular weight 372 aa, 43 kDa
Observed molecular weight 38-42 kDa, 55 kDa
GenBank accession numberBC001968
Gene Symbol CDK9
Gene ID (NCBI) 1025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50750
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243).