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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK9 Recombinant antibody

CDK9 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240600D7

Cat no : 83662-7-RR

Synonyms

CTK1, Cell division cycle 2-like protein kinase 4, CDC2L4, C-2K, C 2k

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK9 Recombinant antibody (83662-7-RR)

    IF Staining of HeLa using 83662-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK9 Recombinant antibody (83662-7-RR)

    IF Staining of HeLa using 83662-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK9 Recombinant antibody (83662-7-RR)

    IF Staining of HeLa using 83662-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83662-7-RR

    Affinity and Kinetic Characterization of 83662-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83662-7-RR against Human CDK9 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83662-7-RR is CDK9 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK9 fusion protein Ag2318 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 9
Calculated molecular weight 372 aa, 43 kDa
Observed molecular weight 38-42 kDa, 55 kDa
GenBank accession numberBC001968
Gene symbol CDK9
Gene ID (NCBI) 1025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243).

Protocols

Product Specific Protocols
IF protocol for CDK9 antibody 83662-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols