• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEACAM1/CD66a Monoclonal antibody

CEACAM1/CD66a Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1H1

Cat no : 68653-1-Ig

Synonyms

CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CEACAM1/CD66a Monoclonal antibody (68653-1-Ig)

    WB analysis using 68653-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68653-1-Ig (CEACAM1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CEACAM1/CD66a Monoclonal antibody (68653-1-Ig)

    IF Staining of HT-29 using 68653-1-Ig

    Immunofluorescent analysis of unfixed HT-29 cells using CEACAM1/CD66a antibody (68653-1-Ig, Clone: 1D1H1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CEACAM1/CD66a Monoclonal antibody (68653-1-Ig)

    IF Staining of HT-29 using 68653-1-Ig

    Immunofluorescent analysis of unfixed HT-29 cells using CEACAM1/CD66a antibody (68653-1-Ig, Clone: 1D1H1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inHepG2 cells, HT-29 cells
Positive IF/ICC detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68653-1-Ig targets CEACAM1/CD66a in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Calculated molecular weight 526 aa, 58 kDa
Observed molecular weight120-150 kDa
GenBank accession numberBC014473
Gene symbol CEACAM1
Gene ID (NCBI) 634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

Protocols

Product Specific Protocols
WB protocol for CEACAM1/CD66a antibody 68653-1-IgDownload protocol
IF protocol for CEACAM1/CD66a antibody 68653-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols