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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEACAM1/CD66a Monoclonal antibody (68653-1-Ig)

    WB analysis using 68653-1-Ig (same clone as 68653-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68653-1-Ig (CEACAM1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68653-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CEACAM1/CD66a Monoclonal antibody (68653-1-Ig)

    IF Staining of HT-29 using 68653-1-Ig (same clone as 68653-1-PBS)

    Immunofluorescent analysis of unfixed HT-29 cells using CEACAM1/CD66a antibody (68653-1-Ig, Clone: 1D1H1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68653-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using CEACAM1/CD66a Monoclonal antibody (68653-1-Ig)

    IF Staining of HT-29 using 68653-1-Ig (same clone as 68653-1-PBS)

    Immunofluorescent analysis of unfixed HT-29 cells using CEACAM1/CD66a antibody (68653-1-Ig, Clone: 1D1H1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68653-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using CEACAM1/CD66a Monoclonal antibody (68653-1-Ig)

    FC experiment of HT-29 using 68653-1-Ig (same clone as 68653-1-PBS)

    1x10^6 HT-29 cells were surface stained with 0.2 μg CEACAM1/CD66a Monoclonal antibody (68653-1-Ig, Clone:1D1H1) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3) was parallel stained as control. Cells were not fixed. This data was developed using the same antibody clone with 68653-1-PBS in a different storage buffer formulation.

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CEACAM1/CD66a Monoclonal antibody, PBS Only

CEACAM1/CD66a Monoclonal Antibody for WB, IF/ICC, FC, Indirect ELISA
Cat No. 68653-1-PBS
Clone No.1D1H1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC, Indirect ELISA

Synonyms

CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68653-1-PBS targets CEACAM1/CD66a in WB, IF/ICC, FC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Calculated molecular weight 526 aa, 58 kDa
Observed molecular weight120-150 kDa
GenBank accession numberBC014473
Gene Symbol CEACAM1
Gene ID (NCBI) 634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13688
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).