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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEACAM1 Recombinant antibody

CEACAM1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240497B4

Cat no : 83524-3-RR

Synonyms

BGP, BGP 1, BGP1, BGPI, Biliary glycoprotein 1, CD66a, CEACAM1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEACAM1 Recombinant antibody (83524-3-RR)

    WB analysis using 83524-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83524-3-RR (CEACAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83524-3-RR is CEACAM1 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Calculated molecular weight 526 aa, 58 kDa
Observed molecular weight135-150 kDa
GenBank accession numberBC014473
Gene symbol CEACAM1
Gene ID (NCBI) 634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

Protocols

Product Specific Protocols
WB protocol for CEACAM1 antibody 83524-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols