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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CEACAM5/8 (CD66b/e) Rabbit Recombinant Antibody

CEACAM5/8 (CD66b/e) Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

230353B7

Cat no : 98012-1-RR

Synonyms

CD66e, CEACAM5, CEACAM5/CD66e, CEACAM8/CD66b, CD66b

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CEACAM5/8 (CD66b/e) Rabbit Recombinant (98012-1-RR)

    FC experiment of human peripheral blood leukocytes using 98012-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CEACAM5/8 (CD66b/e) Rabbit Recombinant Antibody (98012-1-RR, Clone:230353B7) or 0.25 ug Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98012-1-RR

    Affinity and Kinetic Characterization of 98012-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98012-1-RR against Human CEACAM5/8 (CD66b/e) were performed. The affinity constant is 2.31 nM.

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Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98012-1-RR targets CEACAM5/8 (CD66b/e) in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 8
Calculated molecular weight 38 kDa
GenBank accession numberBC026263
Gene symbol CEACAM8
Gene ID (NCBI) 1088
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily of cell adhesion molecules. CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in controlling cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e mainly serves as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEACAM8, also known as CD66b, CGM6 or NCA-95, is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes and is involved in regulating adhesion and activation of eosinophils (PMID: 8104998; 18056392; 18056392). This antibody is raised against 35-319 aa of human CEACAM8/CD66b and can cross-react with CEACAM5/CD66e.

Protocols

Product Specific Protocols
FC protocol for CEACAM5/8 (CD66b/e) antibody 98012-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols