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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Biotin Anti-Human CEACAM5/8 (CD66b/e) Rabbit Recom (Biotin-98012)

    FC experiment of human peripheral blood leukocytes using Biotin-98012

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Biotin Plus Anti-Human CEACAM5/8 (CD66b/e) Rabbit Recombinant Antibody (Biotin-98012, Clone: 230353B7) and CoraLite®488-conjugated streptavidin, or unstained. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Biotin Anti-Human CEACAM5/8 (CD66b/e) Rabbit Recombinant Antibody

CEACAM5/8 (CD66b/e) Uni-rAbTM Recombinant Antibody for FC
Cat No. Biotin-98012
Clone No.230353B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD66b, CEACAM8, CD 67, CD 66b, Carcinoembryonic antigen-related cell adhesion molecule 8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98012 targets CEACAM5/8 (CD66b/e) in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 8
Calculated molecular weight 38 kDa
GenBank accession numberBC026263
Gene Symbol CEACAM8
Gene ID (NCBI) 1088
Conjugate Biotin
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31997
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily of cell adhesion molecules. CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in controlling cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e mainly serves as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEACAM8, also known as CD66b, CGM6 or NCA-95, is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes and is involved in regulating adhesion and activation of eosinophils (PMID: 8104998; 18056392; 18056392). This antibody is raised against 35-319 aa of human CEACAM8/CD66b and can cross-react with CEACAM5/CD66e.

Protocols

Product Specific Protocols
FC protocol for Biotin CEACAM5/8 (CD66b/e) antibody Biotin-98012Download protocol
Standard Protocols
Click here to view our Standard Protocols