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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using FcZero-rAb™ PE Anti-Human CEACAM5/8 (CD66b/e) Rabb (PE-FcA98012)

    FC experiment of human peripheral blood leukocyte using PE-FcA98012

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human CEACAM5/8 (CD66b/e) Rabbit Recombinant Antibody (PE-FcA98012, Clone: 230353B7), or 5 ul PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

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FcZero-rAb™ PE Anti-Human CEACAM5/8 (CD66b/e) Rabbit Recombinant Antibody

CEACAM5/8 (CD66b/e) FcZero-rAbTM Recombinant Antibody for FC
Cat No. PE-FcA98012
Clone No.230353B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD66e, CEACAM5, CD66b, CEACAM8, CD 67

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  PE
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98012 targets CEACAM5/8 (CD66b/e) in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 8
Calculated molecular weight 38 kDa
GenBank accession numberBC026263
Gene Symbol CEACAM8
Gene ID (NCBI) 1088
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily of cell adhesion molecules. CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in controlling cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e mainly serves as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEACAM8, also known as CD66b, CGM6 or NCA-95, is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes and is involved in regulating adhesion and activation of eosinophils (PMID: 8104998; 18056392; 18056392). This antibody is raised against 35-319 aa of human CEACAM8/CD66b and can cross-react with CEACAM5/CD66e.

Protocols

Product Specific Protocols
FC protocol for PE CEACAM5/8 (CD66b/e) antibody PE-FcA98012Download protocol
Standard Protocols
Click here to view our Standard Protocols