CoraLite® Plus 488-conjugated CENPF Polyclonal antibody
CENPF Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-28568
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | A549 cells, HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-28568 targets CENPF in IF/ICC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CENPF fusion protein Ag29665 相同性解析による交差性が予測される生物種 |
Full Name | centromere protein F, 350/400ka (mitosin) |
Calculated molecular weight | 368 kDa |
Observed molecular weight | 350 kDa |
GenBank accession number | NM_016343 |
Gene symbol | CENPF |
Gene ID (NCBI) | 1063 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 CENPF antibody CL488-28568 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |