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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CENPF Polyclonal antibody

CENPF Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28568

Synonyms

AH antigen, CENF, CENP F, CENPF, Centromere protein F, hcp 1, Kinetochore protein CENPF, Mitosin, PRO1779

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated CENPF Polyclonal ant (CL488-28568)

    IF Staining of A549 using CL488-28568

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 CENPF antibody (CL488-28568) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CENPF Polyclonal ant (CL488-28568)

    IF Staining of HeLa using CL488-28568

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CENPF antibody (CL488-28568) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28568 targets CENPF in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPF fusion protein Ag29665 相同性解析による交差性が予測される生物種
Full Name centromere protein F, 350/400ka (mitosin)
Calculated molecular weight 368 kDa
Observed molecular weight350 kDa
GenBank accession numberNM_016343
Gene symbol CENPF
Gene ID (NCBI) 1063
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CENPF antibody CL488-28568Download protocol
Standard Protocols
Click here to view our Standard Protocols