Validation Data Gallery

Filter: AllWBIF/ICC

WB analysis of Jurkat using 11517-1-AP
WB result of CENPJ antibody (11517-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CENPJ transfected Jurkat cells.
WB analysis of mouse testis using 11517-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 11517-1-AP (CENPJ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 11517-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CENPJ antibody (11517-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HepG2 using 11517-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 11517-1-AP (CENPJ antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L). Red: CL555-phalloidin staining of F-actin. .

Featured Product
KD/KO Validated

CENPJ Polyclonal antibody

CENPJ Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 11517-1-AP

Publications(50)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CPAP, LAG-3-associated protein, Centrosomal P4.1-associated protein, Centromere protein J, CENP-J

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (50)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse testis tissue, Jurkat cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 19 publications below
IHC	See 1 publications below
IF	See 42 publications below

Product Information

11517-1-AP targets CENPJ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CENPJ fusion protein Ag2078 相同性解析による交差性が予測される生物種
Full Name	centromere protein J
Calculated molecular weight	1338 aa, 153 kDa
Observed molecular weight	153 kDa
GenBank accession number	BC024209
Gene Symbol	CENPJ
Gene ID (NCBI)	55835
RRID	AB_2244605
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9HC77
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.

Protocols

Product Specific Protocols
WB protocol for CENPJ antibody 11517-1-AP	Download protocol
IF protocol for CENPJ antibody 11517-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nature TRIM37 controls cancer-specific vulnerability to PLK4 inhibition. Authors - Franz Meitinger View Article
human	IF	Science Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4. Authors - Yao Liang Wong View Article
mouse	WB,IF	Nat Cell Biol The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis. Authors - Huijie Zhao View Article
mouse	WB,IF	Neuron Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate. Authors - Divya Jayaraman View Article
rat	IF	Dev Cell SAS-6 Assembly Templated by the Lumen of Cartwheel-less Centrioles Precedes Centriole Duplication. Authors - Chii Shyang Fong View Article
human	IF	Nat Commun Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Authors - Søs Grønbæk Holdgaard View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 11517-1-AP

WB analysis of mouse testis using 11517-1-AP

IF Staining of HepG2 using 11517-1-AP

IF Staining of HepG2 using 11517-1-AP

CENPJ Polyclonal antibody

CENPJ Polyclonal Antibody for WB, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TRIM37 controls cancer-specific vulnerability to PLK4 inhibition.

Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4.

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

SAS-6 Assembly Templated by the Lumen of Cartwheel-less Centrioles Precedes Centriole Duplication.

Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.