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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CENPJ Polyclonal antibody

CENPJ Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11517

Synonyms

BM032, CENP J, CENPJ, centromere protein J, CPAP, LAG 3 associated protein, LAP, LIP1, LYST interacting protein 1, MCPH6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CENPJ Polyclonal ant (CL488-11517)

    IF Staining of HeLa using CL488-11517

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CENPJ antibody (CL488-11517) at dilution of 1:600.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11517 targets CENPJ in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPJ fusion protein Ag2078 相同性解析による交差性が予測される生物種
Full Name centromere protein J
Calculated molecular weight 1338 aa, 153 kDa
GenBank accession numberBC024209
Gene symbol CENPJ
Gene ID (NCBI) 55835
RRIDAB_2919030
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CENPJ antibody CL488-11517Download protocol
Standard Protocols
Click here to view our Standard Protocols