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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CENPJ Polyclonal antibody (11517-1-AP)

    WB analysis of Jurkat using 11517-1-AP (same clone as 11517-1-PBS)

    WB result of CENPJ antibody (11517-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CENPJ transfected Jurkat cells. This data was developed using the same antibody clone with 11517-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CENPJ Polyclonal antibody (11517-1-AP)

    WB analysis of mouse testis using 11517-1-AP (same clone as 11517-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11517-1-AP (CENPJ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11517-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CENPJ Polyclonal antibody (11517-1-AP)

    IF Staining of HepG2 using 11517-1-AP (same clone as 11517-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CENPJ antibody (11517-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 11517-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CENPJ Polyclonal antibody (11517-1-AP)

    IF Staining of HepG2 using 11517-1-AP (same clone as 11517-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 11517-1-AP (CENPJ antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL555-phalloidin staining of F-actin. . This data was developed using the same antibody clone with 11517-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CENPJ Polyclonal antibody, PBS Only

CENPJ Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 11517-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11517-1-PBS targets CENPJ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPJ fusion protein Ag2078 相同性解析による交差性が予測される生物種
Full Name centromere protein J
Calculated molecular weight 1338 aa, 153 kDa
Observed molecular weight 153 kDa
GenBank accession numberBC024209
Gene Symbol CENPJ
Gene ID (NCBI) 55835
RRIDAB_2244605
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HC77
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.