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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CENPN Polyclonal antibody (16751-1-AP)

    WB analysis of MCF-7 using 16751-1-AP (same clone as 16751-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16751-1-AP (CENPN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16751-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

CENPN Polyclonal antibody, PBS Only

CENPN Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16751-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16751-1-PBS targets CENPN in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPN fusion protein Ag10219 相同性解析による交差性が予測される生物種
Full Name centromere protein N
Calculated molecular weight 339 aa, 40 kDa
Observed molecular weight 40 kDa, 25-30 kDa
GenBank accession numberBC008972
Gene Symbol CENPN
Gene ID (NCBI) 55839
RRIDAB_2878310
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96H22
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CENPN, also named as C16orf60 and ICEN32, is a component of the CENPA-NAC complex. It is required for chromosome congression and efficiently align the chromosomes on a metaphase plate. CENPN has 4 isoforms with MW 24 kDa and 35-41 kDa.