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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CEP135 Monoclonal antibody

CEP135 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C4B7

Cat no : 68715-1-Ig

Synonyms

CEP 135, Centrosomal protein of 135 kDa, centrosomal protein 135kDa, 3C4B7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEP135 Monoclonal antibody (68715-1-Ig)

    WB analysis using 68715-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68715-1-Ig (CEP135 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP135 Monoclonal antibody (68715-1-Ig)

    IF Staining of HeLa using 68715-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP135 antibody (68715-1-Ig, Clone: 3C4B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, U2OS cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68715-1-Ig targets CEP135 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CEP135 fusion protein Ag19122 相同性解析による交差性が予測される生物種
Full Name centrosomal protein 135kDa
Calculated molecular weight 1140 aa, 134 kDa
Observed molecular weight135 kDa
GenBank accession numberBC136535
Gene symbol CEP135
Gene ID (NCBI) 9662
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.

Protocols

Product Specific Protocols
WB protocol for CEP135 antibody 68715-1-IgDownload protocol
IF protocol for CEP135 antibody 68715-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols