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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEP135 Monoclonal antibody (68715-1-Ig)

    WB analysis using 68715-1-Ig (same clone as 68715-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68715-1-Ig (CEP135 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68715-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP135 Monoclonal antibody (68715-1-Ig)

    IF Staining of HeLa using 68715-1-Ig (same clone as 68715-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP135 antibody (68715-1-Ig, Clone: 3C4B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68715-1-PBS in a different storage buffer formulation.

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CEP135 Monoclonal antibody, PBS Only

CEP135 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68715-1-PBS
Clone No.3C4B7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CEP 135, Centrosomal protein of 135 kDa, centrosomal protein 135kDa, 3C4B7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68715-1-PBS targets CEP135 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CEP135 fusion protein Ag19122 相同性解析による交差性が予測される生物種
Full Name centrosomal protein 135kDa
Calculated molecular weight 1140 aa, 134 kDa
Observed molecular weight135 kDa
GenBank accession numberBC136535
Gene Symbol CEP135
Gene ID (NCBI) 9662
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ66GS9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.