CEP250,C-NAP1 Monoclonal antibody
CEP250,C-NAP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2G12D12
Cat no : 66814-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
66814-1-Ig targets CEP250,C-NAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CEP250,C-NAP1 fusion protein Ag5925 相同性解析による交差性が予測される生物種 |
| Full Name | centrosomal protein 250kDa |
| Calculated molecular weight | 281 kDa |
| Observed molecular weight | 280 kDa |
| GenBank accession number | BC001433 |
| Gene symbol | CEP250/CNAP1 |
| Gene ID (NCBI) | 11190 |
| RRID | AB_2882157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP250,C-NAP1 antibody 66814-1-Ig | Download protocol |
| IF protocol for CEP250,C-NAP1 antibody 66814-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience Single-cell proteo-genomic reveals a comprehensive map of centrosome-associated spliceosome components | ||
Pancreatology Altered centriolar cohesion by CEP250 and appendages impact outcome of patients with pancreatic cancer |
