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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEP250,C-NAP1 Monoclonal antibody (66814-1-Ig)

    WB analysis using 66814-1-Ig (same clone as 66814-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66814-1-Ig (CEP250,C-NAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEP250,C-NAP1 Monoclonal antibody (66814-1-Ig)

    IF Staining of HepG2 using 66814-1-Ig (same clone as 66814-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEP250,C-NAP1 antibody (66814-1-Ig, Clone: 2G12D12 ) at dilution of 1:773 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

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CEP250,C-NAP1 Monoclonal antibody, PBS Only

CEP250,C-NAP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66814-1-PBS
Clone No.2G12D12

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CEP250,C-NAP1, CNAP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66814-1-PBS targets CEP250,C-NAP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CEP250,C-NAP1 fusion protein Ag5925 相同性解析による交差性が予測される生物種
Full Name centrosomal protein 250kDa
Calculated molecular weight 281 kDa
Observed molecular weight 280 kDa
GenBank accession numberBC001433
Gene Symbol CEP250/CNAP1
Gene ID (NCBI) 11190
RRIDAB_2882157
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BV73
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.