Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-16268 targets CEP63 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.
| Tested Reactivity | human, mouse, rat, Canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CEP63 fusion protein Ag9335 相同性解析による交差性が予測される生物種 |
| Full Name | centrosomal protein 63kDa |
| Calculated molecular weight | 703 aa, 81 kDa |
| Observed molecular weight | 57-75 kDa |
| GenBank accession number | BC014050 |
| Gene Symbol | CEP63 |
| Gene ID (NCBI) | 80254 |
| RRID | AB_2923742 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96MT8 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CEP63 antibody CL488-16268 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |