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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CEP63 Polyclonal antibody

CEP63 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16268

Synonyms

centrosomal protein 63kDa, Centrosomal protein of 63 kDa, CEP63, FLJ13386

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated CEP63 Polyclonal ant (CL488-16268)

    IF Staining of MDCK using CL488-16268

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 488 CEP63 antibody (CL488-16268) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16268 targets CEP63 in IF/ICC applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP63 fusion protein Ag9335 相同性解析による交差性が予測される生物種
Full Name centrosomal protein 63kDa
Calculated molecular weight 703 aa, 81 kDa
Observed molecular weight 57-75 kDa
GenBank accession numberBC014050
Gene symbol CEP63
Gene ID (NCBI) 80254
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP63 is a 63 kDa centrosomal protein. It is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response. This antibody can recognize all the four isoforms of CEP63 (56 kDa, 58 kDa, 63 kDa and 81 kDa).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CEP63 antibody CL488-16268Download protocol
Standard Protocols
Click here to view our Standard Protocols