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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Centrin 1 Polyclonal antibody

Centrin 1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12794-1-AP

Synonyms

CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HL-60 cells using Centrin 1 Polyclonal antibody (12794-1-AP)

    WB analysis of HL-60 using 12794-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 12794-1-AP (Centrin 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using Centrin 1 Polyclonal antibody (12794-1-AP)

    WB analysis of HL-60 using 12794-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 12794-1-AP (Centrin 1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Centrin 1 Polyclonal antibody (12794-1-AP)

    WB analysis of human heart using 12794-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12794-1-AP (Centrin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Centrin 1 Polyclonal antibody (12794-1-AP)

    IF Staining of hTERT-RPE1 using 12794-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Centrin 1 antibody (12794-1-AP) at dilution of 1:50 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L), CoraLite®488 ARL13B antibody (CL488-17711, green), SCLT1 antibody (14875-1-AP, Magenta).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Centrin 1 Polyclonal antibody (12794-1-AP)

    IF Staining of HeLa using 12794-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Centrin 1 antibody (12794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Centrin 1 Polyclonal antibody (12794-1-AP)

    IF Staining of hTERT-RPE1 using 12794-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Centrin 1 antibody (12794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Centrin 1 Polyclonal antibody (12794-1-AP)

    IF Staining of hTERT-RPE1 using 12794-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using Centrin 1 antibody (12794-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Centrin 1 Polyclonal antibody (12794-1-AP)

    IF Staining of HeLa using 12794-1-AP

    Immunofluorescent analysis of Hela cells, using CETN1 antibody 12794-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHL-60 cells, human heart tissue
Positive IF/ICC detected inhTERT-RPE1 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12794-1-AP targets Centrin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Centrin 1 fusion protein Ag3529 相同性解析による交差性が予測される生物種
Full Name centrin, EF-hand protein, 1
Calculated molecular weight 172 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC029515
Gene symbol Centrin 1
Gene ID (NCBI) 1068
RRIDAB_2077371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Protocols

Product Specific Protocols
WB protocol for Centrin 1 antibody 12794-1-APDownload protocol
IF protocol for Centrin 1 antibody 12794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB,IF

Nat Cell Biol

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Authors - Huijie Zhao
View Article
human

Neuron

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Authors - Andrew Kodani
View Article
humanIF

Cell Res

NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.

Authors - Cheng Zhang
View Article
mouseIF

Nat Commun

Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.

Authors - Munemasa Mori
View Article
zebrafishIF

Nucleic Acids Res

Resting cells rely on the DNA helicase component MCM2 to build cilia.

Authors - Teresa Casar Tena
View Article
humanIF

Nat Commun

DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.

Authors - Xiaowei Xu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Julie (Verified Customer) (02-14-2019)

Nice staining of ciliated human fibroblasts.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: human fibroblasts
Centrin 1 Antibody Immunofluorescence, validation (1:100 dilution) in human fibroblasts (Cat no:12794-1-AP)