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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite®594-conjugated Centrin 1 Polyclonal antib (CL594-12794)

    IF Staining of hTERT-RPE1 using CL594-12794

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite®594 Centrin 1 antibody (CL594-12794) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Centrin 1 Polyclonal antib (CL594-12794)

    IF Staining of HeLa using CL594-12794

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 Centrin 1 antibody (CL594-12794) at dilution of 1:200.

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CoraLite®594-conjugated Centrin 1 Polyclonal antibody

Centrin 1 Polyclonal Antibody for IF/ICC
Cat No. CL594-12794

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-12794 targets Centrin 1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Centrin 1 fusion protein Ag3529 相同性解析による交差性が予測される生物種
Full Name centrin, EF-hand protein, 1
Calculated molecular weight 172 aa, 20 kDa
GenBank accession numberBC029515
Gene Symbol Centrin 1
Gene ID (NCBI) 1068
RRIDAB_2919788
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDQ12798
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Protocols

Product Specific Protocols
IF protocol for CL594 Centrin 1 antibody CL594-12794Download protocol
Standard Protocols
Click here to view our Standard Protocols