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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Centrin 1 Monoclonal antibody

Centrin 1 Monoclonal Antibody for IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8D10

Cat no : 68433-1-Ig

Synonyms

Caltractin isoform 2, CEN1, Centrin 1, centrin, EF hand protein, 1, CETN, CETN1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Centrin 1 Monoclonal antibody (68433-1-Ig)

    IF Staining of hTERT-RPE1 using 68433-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using Centrin 1 Monoclonal antibody (68433-1-Ig)

    IF Staining of ARPE-19 using 68433-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Centrin 1 Monoclonal antibody (68433-1-Ig)

    IF Staining of hTERT-RPE1 using 68433-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells, ARPE-19 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68433-1-Ig targets Centrin 1 in IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Centrin 1 fusion protein Ag3529 相同性解析による交差性が予測される生物種
Full Name centrin, EF-hand protein, 1
Calculated molecular weight 172 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC029515
Gene symbol Centrin 1
Gene ID (NCBI) 1068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Protocols

Product Specific Protocols
IF protocol for Centrin 1 antibody 68433-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols