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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Centrin 1 Monoclonal antibody

Centrin 1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G8D10

Cat no : CL488-68433

Synonyms

CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated Centrin 1 Monoclonal (CL488-68433)

    IF Staining of hTERT-RPE1 using CL488-68433

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 Centrin 1 antibody (CL488-68433, Clone: 1G8D10 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated Centrin 1 Monoclonal (CL488-68433)

    IF Staining of hTERT-RPE1 using CL488-68433

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 Centrin 1 antibody (CL488-68433, Clone: 1G8D10 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

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Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68433 targets Centrin 1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Centrin 1 fusion protein Ag3529 相同性解析による交差性が予測される生物種
Full Name centrin, EF-hand protein, 1
Calculated molecular weight 172 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC029515
Gene symbol Centrin 1
Gene ID (NCBI) 1068
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Centrin 1 antibody CL488-68433Download protocol
Standard Protocols
Click here to view our Standard Protocols