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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated Centrin 1 Monoclonal (CL488-68433)

    IF Staining of hTERT-RPE1 using CL488-68433

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 Centrin 1 antibody (CL488-68433, Clone: 1G8D10 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CoraLite® Plus 488-conjugated Centrin 1 Monoclonal (CL488-68433)

    IF Staining of hTERT-RPE1 using CL488-68433

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 Centrin 1 antibody (CL488-68433, Clone: 1G8D10 ) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

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CoraLite® Plus 488-conjugated Centrin 1 Monoclonal antibody

Centrin 1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68433
Clone No.1G8D10

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Synonyms

CETN1, CETN, Centrin-1, CEN1, Caltractin isoform 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68433 targets Centrin 1 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Centrin 1 fusion protein Ag3529 相同性解析による交差性が予測される生物種
Full Name centrin, EF-hand protein, 1
Calculated molecular weight 172 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC029515
Gene Symbol Centrin 1
Gene ID (NCBI) 1068
RRIDAB_3673014
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12798
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Centrin 1 antibody CL488-68433Download protocol
Standard Protocols
Click here to view our Standard Protocols