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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

Cofilin Polyclonal antibody

Cofilin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10960-1-AP

Synonyms

CFL1, 18 kDa phosphoprotein, Cofilin-1, Cofilin1, Cofilin, non-muscle isoform

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cofilin Polyclonal antibody (10960-1-AP)

    WB analysis using 10960-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10960-1-AP (Cofilin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skin tissue using Cofilin Polyclonal antibody (10960-1-AP)

    WB analysis of human skin using 10960-1-AP

    human skin tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using Cofilin Polyclonal antibody (10960-1-AP)

    WB analysis of rat brain using 10960-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (Cofilin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Cofilin Polyclonal antibody (10960-1-AP)

    WB analysis of human brain using 10960-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Cofilin Polyclonal antibody (10960-1-AP)

    WB analysis of mouse brain using 10960-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Cofilin Polyclonal antibody (10960-1-AP)

    WB analysis of MCF-7 using 10960-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Cofilin Polyclonal antibody (10960-1-AP)

    WB analysis of NIH/3T3 using 10960-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cofilin Polyclonal antibody (10960-1-AP)

    IHC staining of human cervical cancer using 10960-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cofilin Polyclonal antibody (10960-1-AP)

    IHC staining of human cervical cancer using 10960-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Cofilin Polyclonal antibody (10960-1-AP)

    IHC staining of human colon using 10960-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10960-1-AP (Cofilin antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cofilin Polyclonal antibody (10960-1-AP)

    IHC staining of human cervical cancer using 10960-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Cofilin Polyclonal antibody (10960-1-AP)

    IHC staining of human cervical cancer using 10960-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:25 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cofilin Polyclonal antibody (10960-1-AP)

    IHC staining of human heart using 10960-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cofilin Polyclonal antibody (10960-1-AP)

    IHC staining of human heart using 10960-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cofilin Polyclonal antibody (10960-1-AP)

    IHC staining of human lung cancer using 10960-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Cofilin Polyclonal antibody (10960-1-AP)

    IHC staining of human lung cancer using 10960-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Cofilin Polyclonal antibody (10960-1-AP)

    IF Staining of NIH/3T3 using 10960-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Cofilin antibody (10960-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Cofilin Polyclonal antibody (10960-1-AP)

    IF Staining of NIH/3T3 using 10960-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 10960-1-AP (Cofilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, NIH/3T3 cells, human skin tissue, human brain tissue, mouse brain tissue, rat brain tissue, NCI-H1299 cells
Positive IHC detected inhuman colon tissue, human cervical cancer tissue, human heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10960-1-AP targets Cofilin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cofilin fusion protein Ag1399 相同性解析による交差性が予測される生物種
Full Name cofilin 1 (non-muscle)
Calculated molecular weight 18 kDa
Observed molecular weight 18-22 kDa
GenBank accession numberBC012318
Gene symbol Cofilin
Gene ID (NCBI) 1072
RRIDAB_2291830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for Cofilin antibody 10960-1-APDownload protocol
IHC protocol for Cofilin antibody 10960-1-APDownload protocol
IF protocol for Cofilin antibody 10960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF,IP

Nat Commun

N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization

Authors - Weiji Weng
View Article
humanWB,IF

Cell Res

NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.

Authors - Cheng Zhang
  • KD Validated
View Article
humanWB

Int J Mol Sci

Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks.

Authors - Marina Damato
View Article
mouseWB

Commun Biol

RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling

Authors - Haixia Zhu
View Article
chickenWB

Microbiol Spectr

Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.

Authors - Beibei Zhang
View Article
humanIHC

Oncotarget

A three-protein signature and clinical outcome in esophageal squamous cell carcinoma.

Authors - Hui-Hui Cao
View Article