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KD/KO Validated

Cofilin Polyclonal antibody

Name: Cofilin Polyclonal antibody
Brand: Proteintech
SKU: 10960-1-AP

Cofilin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10960-1-AP

Synonyms

CFL1, 18 kDa phosphoprotein, Cofilin-1, Cofilin1, Cofilin, non-muscle isoform

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 10960-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10960-1-AP (Cofilin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human skin using 10960-1-AP
human skin tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10960-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10960-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10960-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10960-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (CFL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10960-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10960-1-AP (Cofilin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 10960-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 10960-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 10960-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10960-1-AP (Cofilin antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10960-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 10960-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 10960-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 10960-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 10960-1-AP (CFL1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 10960-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:25 (under 10x lens).
IHC staining of human cervical cancer using 10960-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10960-1-AP (CFL1 antibody) at dilution of 1:25 (under 40x lens).
IF Staining of NIH/3T3 using 10960-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 10960-1-AP (Cofilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of NIH/3T3 using 10960-1-AP
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Cofilin antibody (10960-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 10960-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF-7 cells, NIH/3T3 cells, human skin tissue, human brain tissue, mouse brain tissue, rat brain tissue, NCI-H1299 cells
Positive IHC detected in	human colon tissue, human cervical cancer tissue, human heart tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:600-1:2400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 22 publications below
IHC	See 3 publications below
IF	See 7 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10960-1-AP targets Cofilin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cofilin fusion protein Ag1399 相同性解析による交差性が予測される生物種
Full Name	cofilin 1 (non-muscle)
Calculated molecular weight	18 kDa
Observed molecular weight	18-22 kDa
GenBank accession number	BC012318
Gene symbol	Cofilin
Gene ID (NCBI)	1072
RRID	AB_2291830
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for Cofilin antibody 10960-1-AP	Download protocol
IHC protocol for Cofilin antibody 10960-1-AP	Download protocol
IF protocol for Cofilin antibody 10960-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB,IF,IP	Nat Commun N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization Authors - Weiji Weng View Article
human	WB,IF	Cell Res NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1. Authors - Cheng Zhang KD Validated View Article
human	WB	Int J Mol Sci Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks. Authors - Marina Damato View Article
mouse	WB	Commun Biol RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling Authors - Haixia Zhu View Article
chicken	WB	Microbiol Spectr Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization. Authors - Beibei Zhang View Article
human	IHC	Oncotarget A three-protein signature and clinical outcome in esophageal squamous cell carcinoma. Authors - Hui-Hui Cao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cofilin Polyclonal antibody

Cofilin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 10960-1-AP

WB analysis of human skin using 10960-1-AP

WB analysis of human brain using 10960-1-AP

WB analysis of MCF-7 using 10960-1-AP

WB analysis of NIH/3T3 using 10960-1-AP

WB analysis of mouse brain using 10960-1-AP

WB analysis of rat brain using 10960-1-AP

IHC staining of human cervical cancer using 10960-1-AP

IHC staining of human cervical cancer using 10960-1-AP

IHC staining of human colon using 10960-1-AP

IHC staining of human lung cancer using 10960-1-AP

IHC staining of human lung cancer using 10960-1-AP

IHC staining of human heart using 10960-1-AP

IHC staining of human heart using 10960-1-AP

IHC staining of human cervical cancer using 10960-1-AP

IHC staining of human cervical cancer using 10960-1-AP

IF Staining of NIH/3T3 using 10960-1-AP

IF Staining of NIH/3T3 using 10960-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization

NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.

Protein Kinase C Activation Drives a Differentiation Program in an Oligodendroglial Precursor Model through the Modulation of Specific Biological Networks.

RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling

Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.

A three-protein signature and clinical outcome in esophageal squamous cell carcinoma.

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