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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cofilin Polyclonal antibody

Cofilin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10960

Synonyms

18 kDa phosphoprotein, CFL, CFL1, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18

Validation Data Gallery

Applications:
  • IF Staining of NIH/3T3 using CL488-10960

    IF Staining of NIH/3T3 using CL488-10960

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 Cofilin antibody (CL488-10960) at dilution of 1:200.

Tested Applications

Positive IF detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CFL1 Ready-To-Use IHC Kit. CFL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10960-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-10960 targets Cofilin in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cofilin fusion protein Ag1399 相同性解析による交差性が予測される生物種
Full Name cofilin 1 (non-muscle)
Calculated molecular weight 18 kDa
Observed molecular weight 18-22 kDa
GenBank accession numberBC012318
Gene symbol CFL1
Gene ID (NCBI) 1072
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Cofilin antibody CL488-10960Download protocol
Standard Protocols
Click here to view our Standard Protocols