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CFLAR/FLIP Polyclonal antibody

Name: CFLAR/FLIP Polyclonal antibody
Brand: Proteintech
SKU: 10394-1-AP

CFLAR/FLIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10394-1-AP

Synonyms

CFLAR, CASP8 and FADD-like apoptosis regulator, CASH, c FLIPS, c FLIPR

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of Jurkat using 10394-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10394-1-AP
WB result of CFLAR/FLIP antibody (10394-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CFLAR/FLIP transfected HepG2 cells.
WB analysis of HeLa using 10394-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 10394-1-AP
IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000ug.
IP experiment of Jurkat using 10394-1-AP
IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000ug.
IHC staining of human skeletal muscle using 10394-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10394-1-AP (CFLAR/FLIP antibody at dilution of 1:50.
IHC staining of mouse skeletal muscle using 10394-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 10394-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10394-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CFLAR/FLIP antibody (10394-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 10394-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HepG2 cells, HeLa cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	mouse skeletal muscle tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 1 publications below

Product Information

10394-1-AP targets CFLAR/FLIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CFLAR/FLIP fusion protein Ag0593 相同性解析による交差性が予測される生物種
Full Name	CASP8 and FADD-like apoptosis regulator
Calculated molecular weight	52 kDa
Observed molecular weight	20-35 kDa, 50-55 kDa
GenBank accession number	BC001602
Gene symbol	FLIP
Gene ID (NCBI)	8837
RRID	AB_2081087
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CFLAR, also named CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP, and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein that may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6-mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full-length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.

Protocols

Product Specific Protocols
WB protocol for CFLAR/FLIP antibody 10394-1-AP	Download protocol
IHC protocol for CFLAR/FLIP antibody 10394-1-AP	Download protocol
IF protocol for CFLAR/FLIP antibody 10394-1-AP	Download protocol
IP protocol for CFLAR/FLIP antibody 10394-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Res DAB2IP is a bifunctional tumor suppressor that regulates wildtype RAS and inflammatory cascades in KRAS mutant colon cancer Authors - Abigail L Miller View Article
human	WB	Cell Death Differ Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells. Authors - Leah Fitzsimmons View Article
rat	WB	J Cell Mol Med Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats. Authors - Yong Zhang View Article
human	WB,IHC	Biomed Pharmacother Regorafenib suppresses epidermal growth factor receptor signaling-modulated progression of colorectal cancer. Authors - Yu-Chang Liu View Article
human	WB	Oncol Lett YM155 sensitizes HeLa cells to TRAIL-mediated apoptosis via cFLIP and survivin downregulation. Authors - Arun Pandian Chandrasekaran View Article
human	WB	Mol Med Rep Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells. Authors - Zuofei Chi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CFLAR/FLIP Polyclonal antibody

CFLAR/FLIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 10394-1-AP

WB analysis of HepG2 using 10394-1-AP

WB analysis of HeLa using 10394-1-AP

IP experiment of Jurkat using 10394-1-AP

IP experiment of Jurkat using 10394-1-AP

IHC staining of human skeletal muscle using 10394-1-AP

IHC staining of mouse skeletal muscle using 10394-1-AP

IHC staining of mouse skeletal muscle using 10394-1-AP

IF Staining of HeLa using 10394-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

DAB2IP is a bifunctional tumor suppressor that regulates wildtype RAS and inflammatory cascades in KRAS mutant colon cancer

Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells.

Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.

Regorafenib suppresses epidermal growth factor receptor signaling-modulated progression of colorectal cancer.

YM155 sensitizes HeLa cells to TRAIL-mediated apoptosis via cFLIP and survivin downregulation.

Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells.