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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CFLAR/FLIP Polyclonal antibody

CFLAR/FLIP Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10394

Synonyms

c FLIP, c FLIPL, c FLIPR, c FLIPS, CASH, CASP8AP1, Caspase eight related protein, Caspase homolog, Casper, CFLAR, CLARP, FLAME, FLAME 1, FLAME1, FLIP, I FLICE, Inhibitor of FLICE, MRIT, Usurpin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CFLAR/FLIP Polyclona (CL488-10394)

    IF Staining of HeLa using CL488-10394

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CFLAR/FLIP antibody (CL488-10394) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10394 targets CFLAR/FLIP in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CFLAR/FLIP fusion protein Ag0593 相同性解析による交差性が予測される生物種
Full Name CASP8 and FADD-like apoptosis regulator
Calculated molecular weight 52 kDa
Observed molecular weight 20-35 kDa, 50-55 kDa
GenBank accession numberBC001602
Gene symbol FLIP
Gene ID (NCBI) 8837
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CFLAR, also named CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP, and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein that may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6-mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full-length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CFLAR/FLIP antibody CL488-10394Download protocol
Standard Protocols
Click here to view our Standard Protocols