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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CHCHD10 Polyclonal antibody

CHCHD10 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25671

Synonyms

CoraLite® Plus 488-conjugated CHCHD10 Polyclonal antibody, Protein N27C7-4, Protein N27C7 4, N27C7 4, Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CHCHD10 Polyclonal a (CL488-25671)

    IF Staining of HeLa using CL488-25671

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CHCHD10 antibody (CL488-25671) at dilution of 1:200, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated CHCHD10 Polyclonal a (CL488-25671)

    FC experiment of HeLa using CL488-25671

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CHCHD10 (CL488-25671) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25671 targets CHCHD10 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHCHD10 fusion protein Ag22598 相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 10
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC065232
Gene symbol CHCHD10
Gene ID (NCBI) 400916
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHCHD10 is a mitochondrial protein that is enriched at cristae junctions in the intermembrane space and is likely involved in mitochondrial genome stability and maintenance of cristae junctions. Mutations in CHCHD10 have recently been described as a cause of frontotemporal dementia (FTD) comorbid with amyotrophic lateral sclerosis (ALS).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CHCHD10 antibody CL488-25671Download protocol
FC protocol for CL Plus 488 CHCHD10 antibody CL488-25671Download protocol
Standard Protocols
Click here to view our Standard Protocols