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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD3 Recombinant antibody (82743-1-RR)

    WB analysis using 82743-1-RR (same clone as 82743-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82743-1-RR (CHCHD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CHCHD3 Recombinant antibody (82743-1-RR)

    WB analysis of HEK-293 using 82743-1-RR (same clone as 82743-1-PBS)

    WB result of CHCHD3 antibody (82743-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HEK-293 cells. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using CHCHD3 Recombinant antibody (82743-1-RR)

    IP experiment of Jurkat using 82743-1-RR (same clone as 82743-1-PBS)

    IP result of anti-CHCHD3 (IP:82743-1-RR, 4ug; Detection:82743-1-RR 1:1000) with Jurkat cells lysate 1600 ug. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHCHD3 Recombinant antibody (82743-1-RR)

    IF Staining of HepG2 using 82743-1-RR (same clone as 82743-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (82743-1-RR, Clone: 4B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82743-1-RR

    Affinity and Kinetic Characterization of 82743-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82743-1-RR against Human CHCHD3 were performed. The affinity constant is 5.97 nM.

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  • Featured Product
  • KD/KO Validated

CHCHD3 Recombinant antibody, PBS Only

CHCHD3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 82743-1-PBS
Clone No.4B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82743-1-PBS targets CHCHD3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHCHD3 fusion protein Ag22579 相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 3
Calculated molecular weight 227 aa, 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC014839
Gene Symbol CHCHD3
Gene ID (NCBI) 54927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NX63
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.