• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CHCHD3 Recombinant antibody

CHCHD3 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4B11

Cat no : 82743-1-RR

Synonyms

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CHCHD3 Recombinant antibody (82743-1-RR)

    WB analysis using 82743-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82743-1-RR (CHCHD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CHCHD3 Recombinant antibody (82743-1-RR)

    WB analysis of HEK-293 using 82743-1-RR

    WB result of CHCHD3 antibody (82743-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHCHD3 Recombinant antibody (82743-1-RR)

    IF Staining of HepG2 using 82743-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (82743-1-RR, Clone: 4B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82743-1-RR

    Affinity and Kinetic Characterization of 82743-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82743-1-RR against Human CHCHD3 were performed. The affinity constant is 5.97 nM.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82743-1-RR targets CHCHD3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHCHD3 fusion protein Ag22579 相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 3
Calculated molecular weight 227 aa, 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC014839
Gene symbol CHCHD3
Gene ID (NCBI) 54927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.

Protocols

Product Specific Protocols
WB protocol for CHCHD3 antibody 82743-1-RRDownload protocol
IF protocol for CHCHD3 antibody 82743-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols