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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHD3 Polyclonal antibody

CHD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25335-1-AP

Synonyms

hZFH, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 3, CHD-3, CHD 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CHD3 Polyclonal antibody (25335-1-AP)

    WB analysis using 25335-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25335-1-AP (CHD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CHD3 Polyclonal antibody (25335-1-AP)

    WB analysis of HEK-293 using 25335-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25335-1-AP (CHD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHD3 Polyclonal antibody (25335-1-AP)

    IHC staining of human kidney using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHD3 Polyclonal antibody (25335-1-AP)

    IHC staining of mouse brain using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHD3 Polyclonal antibody (25335-1-AP)

    IHC staining of mouse brain using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CHD3 Polyclonal antibody (25335-1-AP)

    IHC staining of human colon using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHD3 Polyclonal antibody (25335-1-AP)

    IHC staining of human kidney using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CHD3 Polyclonal antibody (25335-1-AP)

    IHC staining of human colon using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CHD3 Polyclonal antibody (25335-1-AP)

    IHC staining of human colon using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CHD3 Polyclonal antibody (25335-1-AP)

    IHC staining of human ovary using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CHD3 Polyclonal antibody (25335-1-AP)

    IHC staining of human ovary using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CHD3 Polyclonal antibody (25335-1-AP)

    IHC staining of human colon using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25335-1-AP targets CHD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chromodomain helicase DNA binding protein 3
Calculated molecular weight 227 kDa
Observed molecular weight 240 kDa
GenBank accession numberNM_005852
Gene symbol CHD3
Gene ID (NCBI) 1107
RRIDAB_2880033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.

Protocols

Product Specific Protocols
WB protocol for CHD3 antibody 25335-1-APDownload protocol
IHC protocol for CHD3 antibody 25335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development

Authors - Xue Chen
View Article