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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CHD3 Polyclonal antibody

CHD3 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25335

Synonyms

hZFH, EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 3, CHD-3, CHD 3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated CHD3 Polyclonal anti (CL488-25335)

    FC experiment of HEK-293 using CL488-25335

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Chd3 Polyclonal Antibody (CL488-25335)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25335 targets CHD3 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chromodomain helicase DNA binding protein 3
Calculated molecular weight 227 kDa
Observed molecular weight 240 kDa
GenBank accession numberNM_005852
Gene symbol CHD3
Gene ID (NCBI) 1107
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CHD3 antibody CL488-25335Download protocol
Standard Protocols
Click here to view our Standard Protocols