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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CHD4 Monoclonal antibody

CHD4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D9G2

Cat no : CL594-66222

Synonyms

ATP dependent helicase CHD4, CHD 4, CHD4, DKFZp686E06161, Mi 2b, Mi2 BETA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated CHD4 Monoclonal antibody (CL594-66222)

    IF Staining of HeLa using CL594-66222

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 CHD4 antibody (CL594-66222, Clone: 3D9G2 ) at dilution of 1:100. Green: Stain with PF00001, CoraLite®488-Phalloidin.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66222 targets CHD4 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CHD4 fusion protein Ag5729 相同性解析による交差性が予測される生物種
Full Name chromodomain helicase DNA binding protein 4
Calculated molecular weight 1937 aa, 221 kDa
Observed molecular weight 260 kDa
GenBank accession numberBC038596
Gene symbol CHD4
Gene ID (NCBI) 1108
RRIDAB_2919967
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.

Protocols

Product Specific Protocols
IF protocol for CL594 CHD4 antibody CL594-66222Download protocol
Standard Protocols
Click here to view our Standard Protocols