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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using CHI3L1/YKL40 Recombinant antibody (83685-4-RR)

    WB analysis of THP-1 using 83685-4-RR (same clone as 83685-4-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 83685-4-RR (CHI3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83685-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CHI3L1/YKL40 Recombinant antibody (83685-4-RR)

    IHC staining of human ovary cancer using 83685-4-RR (same clone as 83685-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83685-4-RR (CHI3L1/YKL40 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83685-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CHI3L1/YKL40 Recombinant antibody (83685-4-RR)

    IHC staining of human ovary cancer using 83685-4-RR (same clone as 83685-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83685-4-RR (CHI3L1/YKL40 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83685-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CHI3L1/YKL40 Recombinant antibody (83685-4-RR)

    IF Staining of THP-1 using 83685-4-RR (same clone as 83685-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CHI3L1 antibody (83685-4-RR, Clone: 240691F11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83685-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using CHI3L1/YKL40 Recombinant antibody (83685-4-RR)

    FC experiment of THP-1 using 83685-4-RR (same clone as 83685-4-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug CHI3L1 Recombinant antibody (83685-4-RR, Clone:240691F11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83685-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83685-4-RR

    Affinity and Kinetic Characterization of 83685-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83685-4-RR against Human CHI3L1 were performed. The affinity constant is 3.14 nM.

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CHI3L1/YKL40 Recombinant antibody, PBS Only

CHI3L1/YKL40 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83685-4-PBS
Clone No.240691F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

CHI3L1, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39, CGP 39

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83685-4-PBS targets CHI3L1/YKL40 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHI3L1/YKL40 fusion protein Ag33123 相同性解析による交差性が予測される生物種
Full Name chitinase 3-like 1 (cartilage glycoprotein-39)
Calculated molecular weight 383 aa, 43 kDa
Observed molecular weight40-43 kDa
GenBank accession numberBC008568
Gene Symbol CHI3L1
Gene ID (NCBI) 1116
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP36222
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)