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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHI3L1/YKL40 Recombinant antibody

CHI3L1/YKL40 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240691F11

Cat no : 83685-4-RR

Synonyms

CHI3L1, Chitinase-3-like protein 1, Chitinase 3 like protein 1, CGP-39, CGP 39

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using CHI3L1 Recombinant antibody (83685-4-RR)

    WB analysis of THP-1 using 83685-4-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 83685-4-RR (CHI3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CHI3L1 / YKL-40 Recombinant antibody (83685-4-RR)

    IF Staining of THP-1 using 83685-4-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CHI3L1 antibody (83685-4-RR, Clone: 240691F11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of THP-1 cells using CHI3L1 / YKL-40 Recombinant antibody (83685-4-RR)

    FC experiment of THP-1 using 83685-4-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug CHI3L1 Recombinant antibody (83685-4-RR, Clone:240691F11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83685-4-RR

    Affinity and Kinetic Characterization of 83685-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83685-4-RR against Human CHI3L1 were performed. The affinity constant is 3.14 nM.

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Tested Applications

Positive WB detected inTHP-1 cells
Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83685-4-RR is CHI3L1/YKL40 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHI3L1/YKL40 fusion protein Ag33123 相同性解析による交差性が予測される生物種
Full Name chitinase 3-like 1 (cartilage glycoprotein-39)
Calculated molecular weight 383 aa, 43 kDa
Observed molecular weight40-43 kDa
GenBank accession numberBC008568
Gene symbol CHI3L1
Gene ID (NCBI) 1116
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

Protocols

Product Specific Protocols
WB protocol for CHI3L1/YKL40 antibody 83685-4-RRDownload protocol
IF protocol for CHI3L1/YKL40 antibody 83685-4-RRDownload protocol
FC protocol for CHI3L1/YKL40 antibody 83685-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols