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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of NIH/3T3 using 12527-1-AP (same clone as 12527-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of human placenta using 12527-1-AP (same clone as 12527-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of human brain using 12527-1-AP (same clone as 12527-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of human heart using 12527-1-AP (same clone as 12527-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human ileum tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of human ileum using 12527-1-AP (same clone as 12527-1-PBS)

    human ileum tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of human kidney using 12527-1-AP (same clone as 12527-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human liver cancer using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human liver cancer using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human liver using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human liver using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHMP2B Polyclonal antibody (12527-1-AP)

    IF Staining of HepG2 using 12527-1-AP (same clone as 12527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CHMP2B antibody (12527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PFA fixed cells using CHMP2B Polyclonal antibody (12527-1-AP)

    IF Staining of PFA fixed cells using 12527-1-AP (same clone as 12527-1-PBS)

    IF result (endosomal stain) of anti-CHMP2B (12527-1-AP) with the cell (PFA fixed) which has been treated with siRNA for VPS4 by Dr. Philip Woodman. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHMP2B Polyclonal antibody (12527-1-AP)

    IF Staining of HepG2 using 12527-1-AP (same clone as 12527-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using CHMP2B antibody 12527-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12527-1-PBS targets CHMP2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP2B fusion protein Ag3222 相同性解析による交差性が予測される生物種
Full Name chromatin modifying protein 2B
Calculated molecular weight 24 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC001553
Gene Symbol CHMP2B
Gene ID (NCBI) 25978
RRIDAB_10603358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQN3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CHMP2B, chromatin-modifying protein 2b, also named CHMP2.5, VPS2B, and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or are replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, which may be caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.