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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHMP4B Recombinant antibody (81691-2-RR)

    WB analysis using 81691-2-RR (same clone as 81691-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81691-2-RR (CHMP4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81691-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CHMP4B Recombinant antibody (81691-2-RR)

    FC experiment of HepG2 using 81691-2-RR (same clone as 81691-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CHMP4B Recombinant antibody (81691-2-RR, Clone:2N22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81691-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81691-2-RR

    Affinity and Kinetic Characterization of 81691-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81691-2-RR against Human CHMP4B were performed. The affinity constant is 4.59 nM.

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CHMP4B Recombinant antibody, PBS Only

CHMP4B Uni-rAbTM Recombinant Antibody for Indirect ELISA, WB
Cat No. 81691-2-PBS
Clone No.2N22

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Synonyms

hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B, C20orf178

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81691-2-PBS targets CHMP4B in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHMP4B fusion protein Ag4544 相同性解析による交差性が予測される生物種
Full Name chromatin modifying protein 4B
Calculated molecular weight 224 aa, 25 kDa
GenBank accession numberBC033859
Gene Symbol CHMP4B
Gene ID (NCBI) 128866
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H444
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.