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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHMP4B Recombinant antibody

CHMP4B Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2N22

Cat no : 81691-2-RR

Synonyms

hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B, C20orf178

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHMP4B Recombinant antibody (81691-2-RR)

    WB analysis using 81691-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81691-2-RR (CHMP4B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using CHMP4B Recombinant antibody (81691-2-RR)

    FC experiment of HepG2 using 81691-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CHMP4B Recombinant antibody (81691-2-RR, Clone:2N22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 81691-2-RR

    Affinity and Kinetic Characterization of 81691-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81691-2-RR against Human CHMP4B were performed. The affinity constant is 4.59 nM.

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HuH-7 cells, C2C12 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 81691-2-RR is CHMP4B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHMP4B fusion protein Ag4544 相同性解析による交差性が予測される生物種
Full Name chromatin modifying protein 4B
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC033859
Gene symbol CHMP4B
Gene ID (NCBI) 128866
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

Protocols

Product Specific Protocols
WB protocol for CHMP4B antibody 81691-2-RRDownload protocol
FC protocol for CHMP4B antibody 81691-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols