CoraLite® Plus 750-conjugated CHMP4B Recombinant antibody
CHMP4B Recombinant Antibody for WB, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, FC (Intra)
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
2N22
Cat no : CL750-81691-2
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HuH-7 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of CL750-81691-2 is CHMP4B Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CHMP4B fusion protein Ag4544 相同性解析による交差性が予測される生物種 |
| Full Name | chromatin modifying protein 4B |
| Calculated molecular weight | 224 aa, 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC033859 |
| Gene symbol | CHMP4B |
| Gene ID (NCBI) | 128866 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 CHMP4B antibody CL750-81691-2 | Download protocol |
| FC protocol for CL Plus 750 CHMP4B antibody CL750-81691-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
