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  • KD/KO Validated

CHMP7 Polyclonal antibody

CHMP7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16424-1-AP

Synonyms

Chromatin-modifying protein 7, Chromatin modifying protein 7


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Tested Applications

Positive WB detected inHEK-293 cells, HEK-293T cells, mouse spinal cord tissue, rat spinal cord tissue, HeLa cells, Jurkat cells
Positive IHC detected inhuman kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16424-1-AP targets CHMP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHMP7 fusion protein Ag9625 相同性解析による交差性が予測される生物種
Full Name CHMP family, member 7
Calculated molecular weight 453 aa, 51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberBC019110
Gene symbol CHMP7
Gene ID (NCBI) 91782
RRIDAB_2079500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHMP7 antibody 16424-1-APDownload protocol
IHC protocol for CHMP7 antibody 16424-1-APDownload protocol
IF protocol for CHMP7 antibody 16424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival

Authors - Pauline Lascaux
humanWB,IF

Nat Cell Biol

Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.

Authors - Marina Vietri
  • KD Validated
humanWB

Dev Cell

CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.

Authors - Leandro N Ventimiglia
  • KD Validated
humanWB

Dev Cell

The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.

Authors - Samuel S Wallis
  • KD Validated
humanWB

Curr Biol

Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation.

Authors - Yolanda Olmos
  • KD Validated
humanWB,IP

Elife

CDK1 controls CHMP7-dependent nuclear envelope reformation.

Authors - Alberto T Gatta
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Viviana (Verified Customer) (06-01-2022)

Very good to IP endogenous protein from mouse tissue (2 ug for less than 2mg of total protein tested) and also for western blot.

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse embryonic tissue
CHMP7 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in mouse embryonic tissue (Cat no:16424-1-AP)