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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CHOP; GADD153 Recombinant antibody (81462-1-RR)

    WB analysis of HeLa using 81462-1-RR (same clone as 81462-1-PBS)

    Untreated and tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81462-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CHOP; GADD153 Recombinant antibody (81462-1-RR)

    WB analysis of Jurkat using 81462-1-RR (same clone as 81462-1-PBS)

    Untreated and tunicamycin treated Jurkat cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81462-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81462-1-PBS

    Affinity and Kinetic Characterization of 81462-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 81462-1-RR against Human CHOP/GADD153 were performed. The affinity constant is 104 nM.

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CHOP; GADD153 Recombinant antibody, PBS Only

CHOP; GADD153 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 81462-1-PBS
Clone No.1G11

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81462-1-PBS targets CHOP; GADD153 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHOP; GADD153 fusion protein Ag7354 相同性解析による交差性が予測される生物種
Full Name DNA-damage-inducible transcript 3
Calculated molecular weight 19 kDa
Observed molecular weight30 kDa
GenBank accession numberBC003637
Gene Symbol CHOP
Gene ID (NCBI) 1649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35638
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).