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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHOP; GADD153 Recombinant antibody

CHOP; GADD153 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G11

Cat no : 81462-1-RR

Synonyms

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CHOP; GADD153 Recombinant antibody (81462-1-RR)

    WB analysis of HeLa using 81462-1-RR

    Untreated and tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CHOP; GADD153 Recombinant antibody (81462-1-RR)

    WB analysis of Jurkat using 81462-1-RR

    Untreated and tunicamycin treated Jurkat cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 81462-1-RR

    Affinity and Kinetic Characterization of 81462-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81462-1-RR against Human CHOP/GADD153 were performed. The affinity constant is 104 nM.

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Tested Applications

Positive WB detected inTunicamycin treated HeLa cells, Tunicamycin treated Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81462-1-RR targets CHOP; GADD153 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHOP; GADD153 fusion protein Ag7354 相同性解析による交差性が予測される生物種
Full Name DNA-damage-inducible transcript 3
Calculated molecular weight 19 kDa
Observed molecular weight30 kDa
GenBank accession numberBC003637
Gene symbol CHOP
Gene ID (NCBI) 1649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Protocols

Product Specific Protocols
WB protocol for CHOP; GADD153 antibody 81462-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols