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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated CHOP; GADD153 Polycl (CL488-15204)

    IF Staining of HeLa using CL488-15204

    Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using CoraLite® Plus 488 CHOP; GADD153 antibody (CL488-15204) at dilution of 1:200, CL594-Phalloidin (red).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CHOP; GADD153 Polyclonal antibody

CHOP; GADD153 Polyclonal Antibody for IF/ICC
Cat No. CL488-15204

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

CHOP, DDIT3, GADD153, CHOP10, CCAAT/enhancer-binding protein homologous protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inTunicamycin treated HeLa cells

Samples need to be treated with ER stress.

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15204 targets CHOP; GADD153 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHOP; GADD153 fusion protein Ag7354 相同性解析による交差性が予測される生物種
Full Name DNA-damage-inducible transcript 3
Calculated molecular weight 19 kDa
Observed molecular weight30 kDa
GenBank accession numberBC003637
Gene Symbol CHOP
Gene ID (NCBI) 1649
RRIDAB_3672624
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35638
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CHOP; GADD153 antibody CL488-15204Download protocol
Standard Protocols
Click here to view our Standard Protocols