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  • Featured Product
  • KD/KO Validated

CHRNB4 Polyclonal antibody, PBS Only

CHRNB4 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22192-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using CHRNB4 Polyclonal antibody (22192-1-AP)

    WB analysis of SH-SY5Y using 22192-1-AP (same clone as 22192-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CHRNB4 Polyclonal antibody (22192-1-AP)

    WB analysis of HeLa using 22192-1-AP (same clone as 22192-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CHRNB4 Polyclonal antibody (22192-1-AP)

    WB analysis of mouse testis using 22192-1-AP (same clone as 22192-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22192-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CHRNB4 Polyclonal antibody (22192-1-AP)

    FC experiment of SH-SY5Y using 22192-1-AP (same clone as 22192-1-PBS)

    1x10^6 SH-SY5Y cells were surface stained with 0.4 ug CHRNB4 Polyclonal antibody (22192-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed. This data was developed using the same antibody clone with 22192-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22192-1-PBS targets CHRNB4 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRNB4 fusion protein Ag16767 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, beta 4
Calculated molecular weight 498 aa, 56 kDa
Observed molecular weight 48-60 kDa
GenBank accession numberBC096080
Gene symbol CHRNB4
Gene ID (NCBI) 1143
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.