Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, T-47D cells, MCF-7 cells, COLO 320 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 3 publications below |
Product Information
66623-1-Ig targets CHST4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CHST4 fusion protein Ag3987 相同性解析による交差性が予測される生物種 |
| Full Name | carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4 |
| Calculated molecular weight | 370 aa, 43 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC035282 |
| Gene Symbol | CHST4 |
| Gene ID (NCBI) | 10164 |
| RRID | AB_2881983 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8NCG5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHST4 antibody 66623-1-Ig | Download protocol |
| IHC protocol for CHST4 antibody 66623-1-Ig | Download protocol |
| IF protocol for CHST4 antibody 66623-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genomics Proteomics Bioinformatics miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma. | ||
Front Oncol Carbohydrate Sulfotransferase 4 Inhibits the Progression of Hepatitis B Virus-Related Hepatocellular Carcinoma and Is a Potential Prognostic Marker in Several Tumors.
| ||
J Leukoc Biol CHST4 associates with high-abundance immune infiltration in hormone receptor-positive breast cancer |